Sadamori N, Matsui S, Han T, Sandberg A A
Cancer Genet Cytogenet. 1984 Jan;11(1):25-9. doi: 10.1016/0165-4608(84)90094-3.
The mitotic index and number of abnormal metaphases of cells stimulated with the various B-cell polyclonal mitogens (PBA) in six B cell chronic lymphocytic leukemia (B-CLL) cases were evaluated. The PBA included tetradecanoyl-0-phorbol-13-acetate (TPA), staphylococcus bacterial strain Cowan I (Cowan I), pokeweed mitogen (PWM), Epstein-Barr virus (EBV), and lipopolysaccharide W from E. coli 0.55:B5 (LPS). TPA could stimulate only 1 of 12 samples. Even though Cowan I led to a relatively high mitotic index, abnormal clones were inconsistently obtained with this mitogen. PWM, EBV, and LPS appear to be the most desirable activators of B-CLL cells among the PBA used in this study.
评估了6例B细胞慢性淋巴细胞白血病（B-CLL）病例中，用各种B细胞多克隆有丝分裂原（PBA）刺激的细胞的有丝分裂指数和异常中期细胞数量。PBA包括十四酰佛波醇-13-乙酸酯（TPA）、考恩I型葡萄球菌（Cowan I）、商陆有丝分裂原（PWM）、爱泼斯坦-巴尔病毒（EBV）和来自大肠杆菌0.55:B5的脂多糖W（LPS）。TPA仅能刺激12个样本中的1个。尽管Cowan I导致相对较高的有丝分裂指数，但用这种有丝分裂原获得异常克隆的情况并不一致。在本研究使用的PBA中，PWM、EBV和LPS似乎是B-CLL细胞最理想的激活剂。
