Rigaud O, Coppey J, Magdelenat H
Cancer Lett. 1983 Aug;20(1):93-6. doi: 10.1016/0304-3835(83)90192-1.
Clonal growth in anchorage dependent (on plastic) and independent (in soft agar) conditions of A 549 cells from a human alveolar carcinoma by thiazolidine-4-carboxylic acid (thioproline) was determined in order to check whether this drug was able to induce reversion towards a normal phenotype of in vivo growth, as described by Gosalvez in HeLa cells. The colony formation on plastic, the ability to form three-dimensional clones in soft agar and cell division in mass culture, were inhibited at similar rates when the cells were grown in the presence of increasing concentrations of thioproline. The morphology of cell clones arising on plastic in the presence of thioproline did not differ from that of untreated cell clones. This indicated that thioproline was unable to provoke 'reverse' transformation of A 549 cells in vitro.
为了检验噻唑烷 - 4 - 羧酸（硫代脯氨酸）是否能够诱导人肺泡癌A549细胞向体内生长的正常表型逆转，就像戈萨尔韦斯在HeLa细胞中所描述的那样，测定了该细胞在贴壁依赖条件（在塑料上）和非贴壁依赖条件（在软琼脂中）下的克隆生长情况。当细胞在浓度不断增加的硫代脯氨酸存在下生长时，在塑料上的集落形成、在软琼脂中形成三维克隆的能力以及大规模培养中的细胞分裂，均以相似的速率受到抑制。在硫代脯氨酸存在的情况下在塑料上形成的细胞克隆形态与未处理的细胞克隆形态并无差异。这表明硫代脯氨酸在体外无法引发A549细胞的“反向”转化。
