García Cañero R, López-Alarcón L, Veloso J J, del Carmen Guijarro M
Servicio de Bioquímica Experimental, Clínica Puerta de Hierro San Martín de Porres, Madrid, Spain.
Int J Cancer. 1988 Jun 15;41(6):859-62. doi: 10.1002/ijc.2910410615.
L-thioproline (thiazolidine-4-carboxylic acid, L-Tp) reduces DNA synthesis in cultured HeLa cells by about 50% when added to synchronized cultures at the beginning of G1 phase. This inhibition is not observed when the drug is added at mid-S, in G2 or in M. L-Tp almost annuls the burst in 22Na uptake occurring at the beginning of G1 and is as potent an inhibitor as amiloride. It is also able to delay the apparition of the early S-specific cAMP peak and to elevate its intracellular concentration. These results indicate that L-Tp could decrease tumor cell growth by acting at the G1-S boundary.
L-硫代脯氨酸（噻唑烷-4-羧酸，L-Tp）在G1期开始时添加到同步培养的细胞中，可使培养的HeLa细胞中的DNA合成减少约50%。当在S期中期、G2期或M期添加该药物时，未观察到这种抑制作用。L-Tp几乎消除了在G1期开始时出现的22Na摄取高峰，并且是与氨氯吡脒一样有效的抑制剂。它还能够延迟早期S期特异性cAMP峰值的出现并提高其细胞内浓度。这些结果表明，L-Tp可能通过作用于G1-S边界来减少肿瘤细胞的生长。
