Grant C K, Ernisse B J, Pontefract R
Cancer Res. 1984 Feb;44(2):498-502.
Following mitogen stimulation (phytohemagglutinin or concanavalin A), feline lymphocytes were maintained in medium containing feline interleukin 2. Lymphocyte lines derived from the blood of feline leukemia virus-infected cats contained cells 50 to 90% of which expressed virus antigens, but lymphocyte lines derived from uninfected cats remained virus free. Leukemia virus-positive and -negative lymphocyte lines retained total dependence upon the presence of interleukin 2 for finite life spans of 20 to 40 cell divisions. Cell-doubling times and surface properties (membrane immunoglobulin negative, guinea pig red cell rosette positive) of all T-cell lines were similar. All cat lymphocyte lines rapidly developed strong but nonspecific cytotoxic effects against a variety of established cat target lines, including normal and leukemia virus-infected fibroblasts and virus-producing lymphomas. Attempts to infect virus-negative lymphocytes with leukemia virus in vitro produced lines containing 1 to 4% of infected cells; subsequently, this level of infection remained constant. Within observation limits, the characteristics of feline leukemia virus-infected and normal cat T-cells were similar. Leukemia virus infection did not predispose target lymphocytes to exhibit properties in vitro that might be associated with preneoplastic change, such as rapid or infinite cell division or development of independence from interleukin 2 regulation.
在用丝裂原（植物血凝素或刀豆球蛋白A）刺激后，猫淋巴细胞在含有猫白细胞介素2的培养基中培养。从感染猫白血病病毒的猫血液中获得的淋巴细胞系，其细胞中有50%至90%表达病毒抗原，但从未感染猫获得的淋巴细胞系仍无病毒。白血病病毒阳性和阴性淋巴细胞系在20至40次细胞分裂的有限寿命期内，仍然完全依赖白细胞介素2的存在。所有T细胞系的细胞倍增时间和表面特性（膜免疫球蛋白阴性，豚鼠红细胞玫瑰花结阳性）相似。所有猫淋巴细胞系都迅速对多种已建立的猫靶细胞系产生强烈但非特异性的细胞毒性作用，包括正常和感染白血病病毒的成纤维细胞以及产生病毒的淋巴瘤。试图在体外用白血病病毒感染病毒阴性淋巴细胞，产生的细胞系含有1%至4%的感染细胞；随后，这种感染水平保持恒定。在观察范围内，感染猫白血病病毒的猫T细胞和正常猫T细胞的特征相似。白血病病毒感染并未使靶淋巴细胞在体外表现出可能与肿瘤前期变化相关的特性，如快速或无限的细胞分裂或对白细胞介素2调节的独立性发展。
