Cate C C, Douple E B, Andrews K M, Pettengill O S, Curphey T J, Sorenson G D, Maurer L H
Cancer Res. 1984 Mar;44(3):949-54.
A calcitonin (CT)-producing cell line (DMS53) established from human small cell carcinoma of the lung was grown as three-dimensional multicellular spheroids in spinner culture or on agar in multiwells, and as tumors in nude (athymic) mice. CT release into the media was directly proportional to spheroid volume. The response of these cells following exposures to X-irradiation, Adriamycin, or diazoacetylcholine iodide was assessed by monitoring levels of CT released into the media by individual spheroids. Levels of CT in the blood of nude mice bearing DMS53 xenografts were directly proportional to tumor volume and decreased proportionally with tumor response to X-irradiation and cisplatin treatment. These results suggest that the DMS53 spheroid and xenograft models may be useful systems to monitor responses to therapy utilizing CT as an indicator of tumor burden.
从人肺小细胞癌建立的产生降钙素（CT）的细胞系（DMS53），在转瓶培养中或在多孔板的琼脂上作为三维多细胞球体生长，并在裸（无胸腺）小鼠体内形成肿瘤。CT释放到培养基中的量与球体体积成正比。通过监测单个球体释放到培养基中的CT水平，评估这些细胞在暴露于X射线、阿霉素或碘化重氮乙酰胆碱后的反应。携带DMS53异种移植物的裸鼠血液中的CT水平与肿瘤体积成正比，并随着肿瘤对X射线和顺铂治疗的反应成比例下降。这些结果表明，DMS53球体和异种移植模型可能是有用的系统，以利用CT作为肿瘤负荷指标来监测对治疗的反应。
