Sykes S E, Moores S R, Jones S T
Exp Lung Res. 1983 Dec;5(4):245-57. doi: 10.3109/01902148309061518.
The response of the rat lung to a range of doses of quartz at 50 and 100 days after its administration by intratracheal instillation has been assessed by bronchopulmonary lavage. The effects on the levels of protein and hydroxyproline and on the PZ peptidase and collagenolytic activities of lavage fluid supernatants are described and an assessment of the hydroxyproline content of recovered cells is made. In addition, assays for PZ peptidase and collagenase activity were carried out on polymorph enriched fractions and on samples obtained from short-term culture of recovered macrophages. Both protein and hydroxyproline measurements did show dependence on the amount of quartz instilled and the time after its administration. Measurements of PZ peptidase and collagenase activities suggest that the polymorphonuclear leukocytes, not the macrophages, are the major source of these degradative enzymes. The relevance of these findings with regard to the importance of the PMN in quartz-induced fibrosis, is discussed.
通过支气管肺灌洗评估了大鼠经气管内滴注给予一系列剂量石英后50天和100天时肺的反应。描述了对灌洗流体上清液中蛋白质和羟脯氨酸水平以及对PZ肽酶和胶原分解活性的影响，并对回收细胞的羟脯氨酸含量进行了评估。此外，对多形核白细胞富集部分和从回收巨噬细胞的短期培养物中获得的样品进行了PZ肽酶和胶原酶活性测定。蛋白质和羟脯氨酸测量均显示依赖于所滴注石英的量及其给药后的时间。PZ肽酶和胶原酶活性的测量表明，多形核白细胞而非巨噬细胞是这些降解酶的主要来源。讨论了这些发现与多形核白细胞在石英诱导的纤维化中的重要性的相关性。
