Sykes S E, Morgan A, Moores S R, Holmes A, Davison W
Exp Lung Res. 1983 Dec;5(4):229-43. doi: 10.3109/01902148309061517.
The response of the rat lung to a range of doses of quartz at 50 and 100 days after its administration by intratracheal instillation was investigated by bronchopulmonary lavage. The effects on the numbers of polymorphonuclear leucocytes, lymphocytes and macrophages recovered are described. In addition, the size distribution of the recovered macrophages was followed and measurements of total numbers of macrophages in the lung were made using a radioactive tracer technique. Finally, the activity of the cytoplasmic enzyme, lactate dehydrogenase, in lavage fluid supernatants was recorded. There was a dose-dependent increase in the recovery of all three cell types and an increase in the total numbers of macrophages in the lungs which showed no signs of resolution over the 100 day period studied. Increasing levels of lactate dehydrogenase were also dose-dependent and well correlated with the numbers of polymorphs and lymphocytes recovered. The importance of this prolonged inflammatory reaction in relation to the fibrogenic action of quartz is discussed.
通过支气管肺灌洗研究了大鼠经气管内滴注给予一系列剂量石英后50天和100天时肺的反应。描述了对回收的多形核白细胞、淋巴细胞和巨噬细胞数量的影响。此外，跟踪回收巨噬细胞的大小分布，并使用放射性示踪技术测量肺中巨噬细胞的总数。最后，记录灌洗液上清液中细胞质酶乳酸脱氢酶的活性。所有三种细胞类型的回收率均呈剂量依赖性增加，肺中巨噬细胞总数增加，在研究的100天期间没有消退迹象。乳酸脱氢酶水平的升高也呈剂量依赖性，且与回收的多形核细胞和淋巴细胞数量密切相关。讨论了这种长期炎症反应与石英致纤维化作用的关系。
