Eppig J J, Ward-Bailey P F
Exp Cell Res. 1984 Feb;150(2):459-65. doi: 10.1016/0014-4827(84)90590-1.
Prior to ovulation, the cumulus cells that surround the oocyte become embedded in a matrix containing hyaluronic acid (HA). Sulfated glycosaminoglycans (GAGs) prevent the hormonally stimulated deposition of this matrix in vitro. The goal of this project was to determine the effect of sulfated GAGs on the HA-synthesizing activity of the cumuli oophori. This activity was measured in lysates of mouse cumuli oophori after stimulation of isolated cumulus cell-oocyte complexes with follicle-stimulating hormone (FSH) in the presence or absence of sulfated GAGs. FSH treatment resulted in a 5-fold stimulation of HA-synthesizing activity by 3 h in vitro. This induction was inhibited in a dose-dependent manner by heparin and chondroitin sulfate B. However, addition of heparin or chondroitin sulfate B to the assay mixtures containing lysates of FSH-stimulated cumuli oophori had no effect on the HA-synthesizing activity. Heparin also suppressed HA-synthesizing activity stimulated by dibutyryl cyclic adenosine monophosphate. Heparin inhibited the continued increase in hyaluronic acid synthesizing activity when added to cultures after 3 h of FSH stimulation. Also, addition of heparin to cultures of cumuli oophori after 3 or 6 h of incubation in medium containing FSH resulted in only partial cumulus expansion. These results indicate that sulfated GAGs, which are found in ovarian follicular fluid and are a component of extracellular matrix, inhibit some cellular process(es) that results in increased HA-synthesizing activity. The sulfated GAGs also have the ability to suppress HA-synthesizing activity after it has been induced to levels that result in partial cumulus expansion. However, the sulfated GAGs are not direct enzyme inhibitors.
在排卵前，围绕卵母细胞的卵丘细胞会嵌入含有透明质酸（HA）的基质中。硫酸化糖胺聚糖（GAGs）可在体外阻止这种基质的激素刺激沉积。本项目的目的是确定硫酸化GAGs对卵丘透明质酸合成活性的影响。在用或不用硫酸化GAGs的情况下，用促卵泡激素（FSH）刺激分离的卵丘细胞 - 卵母细胞复合体后，在小鼠卵丘细胞裂解物中测量这种活性。FSH处理在体外3小时内使HA合成活性提高了5倍。肝素和硫酸软骨素B以剂量依赖性方式抑制这种诱导作用。然而，向含有FSH刺激的卵丘细胞裂解物的测定混合物中添加肝素或硫酸软骨素B对HA合成活性没有影响。肝素还抑制了二丁酰环磷酸腺苷刺激的HA合成活性。当在FSH刺激3小时后添加到培养物中时，肝素抑制了透明质酸合成活性的持续增加。此外，在含有FSH的培养基中孵育3或6小时后，向卵丘细胞培养物中添加肝素仅导致部分卵丘扩展。这些结果表明，在卵巢卵泡液中发现的、作为细胞外基质成分的硫酸化GAGs抑制了某些导致HA合成活性增加的细胞过程。硫酸化GAGs在HA合成活性被诱导到导致部分卵丘扩展的水平后，也有抑制该活性的能力。然而，硫酸化GAGs不是直接的酶抑制剂。
