Tesarík J, Kopecný V
Histochemistry. 1986;85(6):523-8. doi: 10.1007/BF00508435.
Light- and electron-microscope autoradiography using 3H-glucosamine and 3H-fucose as precursors was employed to investigate proteoglycan synthesis and secretion by late preovulatory human oocytes and cumulus cells. Both the oocyte and cumulus cells were found to be important cellular sources supplying proteoglycans to the oocyte-cumulus-complex extracellular matrices, i.e., the zona pellucida and the cumulus intercellular matrix. Both the oocyte and cumulus cells were shown to secrete labelled proteoglycans into the zona pellucida. Labelled proteoglycans were also detected in the cumulus intercellular matrix. Chase experiments revealed the labelled molecules to be relatively closely associated with both the zona pellucida and the cumulus intercellular matrix. Staining with chromic acid and phosphotungstic acid showed proteoglycan material to penetrate from the cumulus intercellular matrix into pores of the zona pellucida. This material is thought to be a structural equivalent of the newly synthesized proteoglycans secreted by cumulus cells and migrating into the zona pellucida (as detected by autoradiography). It is concluded that newly synthesized proteoglycans secreted by the oocyte and cumulus cells in the late preovulatory period are a component of the microenvironment in which fertilization takes place.
利用³H-葡糖胺和³H-岩藻糖作为前体进行光镜和电镜放射自显影，以研究排卵前晚期人卵母细胞和卵丘细胞的蛋白聚糖合成与分泌。发现卵母细胞和卵丘细胞都是向卵母细胞-卵丘复合体细胞外基质（即透明带和卵丘细胞间基质）供应蛋白聚糖的重要细胞来源。卵母细胞和卵丘细胞均被证明能向透明带分泌标记的蛋白聚糖。在卵丘细胞间基质中也检测到了标记的蛋白聚糖。追踪实验表明，标记分子与透明带和卵丘细胞间基质的结合相对紧密。用铬酸和磷钨酸染色显示，蛋白聚糖物质从卵丘细胞间基质渗入透明带的孔隙。这种物质被认为是卵丘细胞分泌并迁移到透明带中的新合成蛋白聚糖的结构等效物（如放射自显影所检测到的）。得出的结论是，排卵前晚期卵母细胞和卵丘细胞分泌的新合成蛋白聚糖是受精发生的微环境的一个组成部分。
