Bollen A, Loriau R, Herzog A, Hérion P
FEBS Lett. 1984 Jan 23;166(1):67-70. doi: 10.1016/0014-5793(84)80046-0.
Complementary DNA coding for human alpha 1-antitrypsin has been placed under the control of the lambda PR promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human alpha 1-antitrypsin (+/- 9000 molecules/cell). The product has a Mr of 44000, corresponding to mature unglycosylated alpha 1-antitrypsin.
编码人α1 -抗胰蛋白酶的互补DNA已通过表达载体pCQV2置于λPR启动子载体的控制之下[1]。在允许从该启动子转录的条件下（阻遏物的热失活），携带重组质粒pULB1114的大肠杆菌细胞表达人α1 -抗胰蛋白酶（约9000个分子/细胞）。该产物的相对分子质量为44000，对应于成熟的未糖基化α1 -抗胰蛋白酶。
