Wasylyk B, Wasylyk C, Chambon P
Fed Proc. 1984 Feb;43(2):226-34.
Using both clones of mouse LMTK- cells cotransformed with various chimeric conalbumin promoter simian virus 40 (SV40) early gene recombinants and the herpes thymidine kinase gene, and HeLa cells transfected with the same chimeric recombinants, we show that the SV40 72 base pair (bp) repeat sequence is a bidirectional potentiator of initiation of transcription from adjacent T-A-T-A box-dependent and -independent start sites. These results are consistent with our previous model based mainly on the results of T antigen gene expression assays that the 72-bp repeat acts as a bidirectional entry site for RNA polymerase B. We also show that the conalbumin T-A-T-A box is an important element for efficient and accurate in vivo initiation of transcription.
利用与各种嵌合伴清蛋白启动子猿猴病毒40（SV40）早期基因重组体和疱疹胸苷激酶基因共转化的小鼠LMTK-细胞克隆，以及用相同嵌合重组体转染的HeLa细胞，我们表明SV40 72碱基对（bp）重复序列是从相邻T-A-T-A盒依赖性和非依赖性起始位点起始转录的双向增强子。这些结果与我们之前主要基于T抗原基因表达分析结果的模型一致，即72-bp重复序列作为RNA聚合酶B的双向进入位点。我们还表明，伴清蛋白T-A-T-A盒是体内高效准确起始转录的重要元件。
