Miyashiro S, Ando T, Hirayama K, Kida T, Shibai H, Murai A, Shiio T, Udaka S
J Antibiot (Tokyo). 1983 Dec;36(12):1638-43. doi: 10.7164/antibiotics.36.1638.
Two streptothricin-group antibiotics, AN-201 I and II, were newly discovered and isolated from the culture broth of Streptomyces nojiriensis C-13. These antibiotics were purified by IRC-50 (H+) and CM-Sephadex C-50 chromatography, and paper electrophoresis. Structural analysis of AN-201 I and II showed that they were N beta-acetylated derivatives of streptothricin E and D, respectively. They had antibacterial activities against several strains of Escherichia coli, Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus, and showed a strong selective cytotoxic effect on 3T3 cells transformed with SV-40 as compared with their normal cells in a test system in vitro as well as in vivo.
两种链丝菌素类抗生素，AN-201 I和II，是从野尻链霉菌C-13的培养液中首次发现并分离得到的。这些抗生素通过IRC-50（H+）和CM-葡聚糖凝胶C-50柱色谱以及纸电泳进行纯化。对AN-201 I和II的结构分析表明，它们分别是链丝菌素E和D的Nβ-乙酰化衍生物。它们对几种大肠杆菌、枯草芽孢杆菌、藤黄微球菌和金黄色葡萄球菌菌株具有抗菌活性，并且在体外和体内测试系统中，与正常细胞相比，对用SV-40转化的3T3细胞表现出强烈的选择性细胞毒性作用。
