de Groot E J, Schweiger H G
J Cell Sci. 1983 Nov;64:27-36. doi: 10.1242/jcs.64.1.27.
The activity of dTMP kinase (EC 2.7.4.9) was estimated during the development of Acetabularia. Enzyme activity was increased at the beginning of the generative phase. Regulation of the dTMP kinase activity was observed even in the absence of the nucleus. More than 30 days after enucleation the enzyme activity increased two- to threefold. The increase in activity was inhibited by puromycin and cycloheximide but not by rifampicin and chloramphenicol. These results indicate that the enzyme is coded by the nuclear genome and translated on 80 S ribosomes. From mixing experiments with low-activity and high-activity cell extracts it is concluded that the regulation is due to de novo synthesis of the enzyme.
在伞藻发育过程中对胸苷酸激酶（EC 2.7.4.9）的活性进行了评估。在生殖阶段开始时酶活性增加。即使在没有细胞核的情况下也观察到了胸苷酸激酶活性的调节。去核30多天后，酶活性增加了两到三倍。嘌呤霉素和环己酰亚胺抑制活性的增加，但利福平和平氯霉素则没有。这些结果表明该酶由核基因组编码并在80S核糖体上翻译。通过低活性和高活性细胞提取物的混合实验得出结论，这种调节是由于酶的从头合成。
