Mednieks M I, Hand A R
J Dent Res. 1984 Feb;63(2):87-93. doi: 10.1177/00220345840630021601.
Two ultrasensitive methods were employed to determine the effect of repeated in vivo stimulation with isoproterenol on the protein composition of parotid acinar cells. Silver staining showed electrophoretic banding pattern changes in a wide range of cellular proteins during and after multiple injections of the beta-adrenergic agonist. Photo-affinity labeling with [32P]-N3-cyclic AMP showed the disappearance and eventual return (after discontinuation of the injections) of the cellular cyclic AMP-binding proteins as measurable cellular components. These findings indicate that major translation-associated changes may occur in target cell protein composition both during and after hormonal stimulation.
采用两种超灵敏方法来确定用异丙肾上腺素进行体内反复刺激对腮腺腺泡细胞蛋白质组成的影响。银染显示，在多次注射β-肾上腺素能激动剂期间及之后，多种细胞蛋白质的电泳条带模式发生了变化。用[32P]-N3-环磷酸腺苷进行光亲和标记显示，作为可测量的细胞成分，细胞环磷酸腺苷结合蛋白消失，并最终恢复（注射停止后）。这些发现表明，在激素刺激期间及之后，靶细胞蛋白质组成可能会发生与翻译相关的主要变化。
