Gordon I, Stevenson D, Tumas V, Natham C R
J Gen Virol. 1983 Dec;64 ( Pt 12):2777-80. doi: 10.1099/0022-1317-64-12-2777.
The interferon receptors of C3H/10T1/2 mouse cells respond differently to alpha and beta interferons under certain conditions. If C3H/10T1/2 cells which have been maintained in logarithmic growth phase are exposed to trypsin or Pronase immediately before they are treated with mouse interferons, they evince an antiviral response to alpha interferon but not to beta interferon. In contrast, contact-inhibited C3H/10T1/2 cells, L-929 mouse cells or human HEL cells lost the ability to respond to both alpha and beta interferons after treatment with trypsin or Pronase. When L-929 cells are incubated at 37 degrees C following exposure to these proteolytic enzymes, they completely regain their ability to respond to mouse beta interferon within 2 h. These observations suggest that the receptors for alpha and beta interferons are different in their topographical distribution in C3H/10T1/2 cells.
在某些条件下，C3H/10T1/2小鼠细胞的干扰素受体对α干扰素和β干扰素的反应不同。如果处于对数生长期的C3H/10T1/2细胞在接受小鼠干扰素处理之前立即暴露于胰蛋白酶或链霉蛋白酶中，它们会表现出对α干扰素的抗病毒反应，但对β干扰素没有反应。相比之下，接触抑制的C3H/10T1/2细胞、L-929小鼠细胞或人HEL细胞在用胰蛋白酶或链霉蛋白酶处理后失去了对α和β干扰素的反应能力。当L-929细胞在暴露于这些蛋白水解酶后于37℃孵育时，它们在2小时内完全恢复了对小鼠β干扰素的反应能力。这些观察结果表明，α和β干扰素的受体在C3H/10T1/2细胞中的拓扑分布不同。
