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araE低亲和力L-阿拉伯糖转运启动子。调控蛋白的克隆、序列、转录起始位点及DNA结合位点。

The araE low affinity L-arabinose transport promoter. Cloning, sequence, transcription start site and DNA binding sites of regulatory proteins.

作者信息

Stoner C, Schleif R

出版信息

J Mol Biol. 1983 Dec 25;171(4):369-81. doi: 10.1016/0022-2836(83)90035-9.

DOI:10.1016/0022-2836(83)90035-9
PMID:6319708
Abstract

The promoter for the gene encoding the low affinity L-arabinose uptake protein in Escherichia coli was studied. The promoter was cloned, sequenced, its transcription start site determined by S1 nuclease mapping, the proteins required for in vitro transcription were determined, and the regulatory protein binding sites located by DNase footprinting. The araE promoter shows no evidence of an operator site upstream from the CRP binding site, but otherwise it is similar to the araBAD promoter.

摘要

对大肠杆菌中编码低亲和力L-阿拉伯糖摄取蛋白的基因启动子进行了研究。该启动子被克隆、测序，通过S1核酸酶图谱确定其转录起始位点，确定体外转录所需的蛋白质，并通过DNase足迹法定位调控蛋白结合位点。araE启动子在CRP结合位点上游没有操纵位点的证据，但在其他方面与araBAD启动子相似。

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The araE low affinity L-arabinose transport promoter. Cloning, sequence, transcription start site and DNA binding sites of regulatory proteins.araE低亲和力L-阿拉伯糖转运启动子。调控蛋白的克隆、序列、转录起始位点及DNA结合位点。
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