Czarniecki C W, Fennie C W, Powers D B, Estell D A
J Virol. 1984 Feb;49(2):490-6. doi: 10.1128/JVI.49.2.490-496.1984.
The antiviral and antiproliferative effects of highly purified Escherichia coli-derived human interferons (IFNs) were examined in human melanoma cells (Hs294T). Antiproliferative activity was monitored by measuring inhibition of cell multiplication, and antiviral activity was determined by inhibition of herpes simplex virus type 1 replication. Treatment of cells with IFN-gamma in combination with IFN-alpha A or IFN-beta 1 resulted in potentiation of both antiproliferative and antiviral activities. In contrast, combination treatments composed of IFN-alpha A and IFN beta 1 yielded inconsistent results. Some combinations reflected additive responses, whereas others were antagonistic. To examine correlations between IFN-induced biological activities and interactions of the different IFNs with cell surface receptors, in vivo [35S]methionine-labeled IFN-alpha A was prepared. Binding studies indicated the presence of 2,980 +/- 170 receptors per cell, each with an apparent Kd of (8.4 +/- 1.3) X 10(-11) M. Results from competitive binding studies suggested that Hs294T cells possess at least two types of IFN receptors: one which binds IFN-alpha A and IFN-beta 1 and another to which IFN-gamma binds.
在人黑色素瘤细胞（Hs294T）中检测了高度纯化的大肠杆菌衍生的人干扰素（IFN）的抗病毒和抗增殖作用。通过测量细胞增殖抑制来监测抗增殖活性，通过抑制单纯疱疹病毒1型复制来确定抗病毒活性。用γ干扰素与αA干扰素或β1干扰素联合处理细胞导致抗增殖和抗病毒活性均增强。相比之下，由αA干扰素和β1干扰素组成的联合处理产生了不一致的结果。一些组合表现为相加反应，而其他组合则表现为拮抗作用。为了研究IFN诱导的生物学活性与不同IFN与细胞表面受体相互作用之间的相关性，制备了体内[35S]甲硫氨酸标记的αA干扰素。结合研究表明，每个细胞存在2980±170个受体，每个受体的表观解离常数为（8.4±1.3）×10-11M。竞争性结合研究结果表明，Hs294T细胞至少拥有两种类型的IFN受体：一种结合αA干扰素和β1干扰素，另一种结合γ干扰素。
