Hannigan G E, Fish E N, Williams B R
J Biol Chem. 1984 Jul 10;259(13):8084-6.
The human neuroblastoma cell line T98G is sensitive to the antiproliferative activity of both alpha- and gamma-interferons (IFNs). Specific binding of a homogeneous 125I-IFN-alpha 2 probe to high-affinity membrane receptors (apparent Kd = 6.2 X 10(-10) M) can be decreased by pretreatment of these cells with human IFN-gamma (HuIFN-gamma, derived by recombinant DNA techniques). The regulation of HuIFN-alpha 2 binding by such pretreatment is reflected by subsequent inhibition of both the antiviral and antiproliferative activities of HuIFN-alpha 2 on T98G cells. Thus, binding of HuIFN-gamma to its distinct cell-surface receptor can modify expression of the separate IFN-alpha receptor, presumably through an indirect effect on the apparent Kd of the IFN-alpha 2 receptor-ligand interaction.
人神经母细胞瘤细胞系T98G对α-干扰素和γ-干扰素（IFN）的抗增殖活性敏感。用重组DNA技术衍生的人γ-干扰素（HuIFN-γ）预处理这些细胞，可降低均一的125I-IFN-α2探针与高亲和力膜受体（表观解离常数Kd = 6.2×10-10 M）的特异性结合。这种预处理对HuIFN-α2结合的调节反映在随后HuIFN-α2对T98G细胞的抗病毒和抗增殖活性的抑制上。因此，HuIFN-γ与其独特的细胞表面受体的结合可能通过对IFN-α2受体-配体相互作用的表观解离常数的间接影响，来改变单独的IFN-α受体的表达。
