Bloom N D, Johnson F, Pertshuck L, Fishman J
J Surg Oncol. 1984 Jan;25(1):21-4. doi: 10.1002/jso.2930250107.
The determination of steroid receptors in human breast cancers has assumed increasing importance over the past several decades. Improper handling of the specimens could affect results obtained. This study details the effects excessive levels of heat that occur with the use of electrocautery can have on steroid receptor quantities and localization. Twelve resected primary and metastatic human breast cancers were analyzed for cytoplasmic and nuclear receptors by biochemical analysis. In addition, steroid binding was determined by direct fluorescent histochemical techniques. To a portion of each resected specimen a Boviec was applied to simulate electrocautery resection. Analysis of the different portions of the same tumor revealed that there was a decrease in measurable cytoplasmic receptor in all cauterized specimens and a concomitant increase in the nuclear receptor. A similar shift in steroid binding was noted in all the specimens analyzed by fluorescent histochemical techniques. The results of this study show that the application of excessive heat to human breast cancers will lead to false negative biochemical steroid receptor determination by shifting the receptors intranuclear.
在过去几十年中，测定人类乳腺癌中的类固醇受体变得越来越重要。标本处理不当可能会影响所获得的结果。本研究详细阐述了使用电灼时出现的过高温度对类固醇受体数量和定位的影响。通过生化分析对12例切除的原发性和转移性人类乳腺癌进行了细胞质和细胞核受体分析。此外，通过直接荧光组织化学技术测定类固醇结合情况。对每个切除标本的一部分施加博维电刀以模拟电灼切除。对同一肿瘤的不同部分进行分析发现，所有经电灼处理的标本中可测量的细胞质受体减少，同时细胞核受体增加。在所有通过荧光组织化学技术分析的标本中都观察到类固醇结合有类似的变化。本研究结果表明，对人类乳腺癌施加过高温度会通过使受体内移而导致生化类固醇受体测定出现假阴性。
