Alessi N E, Khachaturian H, Watson S, Akil H
Life Sci. 1983;33 Suppl 1:57-60. doi: 10.1016/0024-3205(83)90443-5.
Extracts of the anterior lobe and intermediate lobe of postnatal (P) (Day P1, P7, P14, P21, P28, P35, P42) and adult male Sprague-Dawley rats were analyzed by both a Beta-endorphin (B-END) radioimmunoassay and a radioimmunoassay for N-acetyl-B-END. In the anterior lobe, on P1, less than 2% of the adult level of B-END was present. By P42 this level had increased to 21% of adult levels. In the intermediate lobe, on P1, the B-END levels were less than 0.1% of the adult level, and by P42 this level approached approximately 45% of the adult levels. N-acetylated B-END was identified in both anterior lobe and intermediate lobe from P1 through adulthood. In the anterior lobe at P1, N-acetyl-B-END immunoreactivity contributes approximately 25% of the total B-END immunoreactivity. This level drops to less than 10% by P21, and to adult-like levels by P42 (less than 5%). On the other hand, in the intermediate lobe, the N-acetyl-B-END levels start at 70% of the total B-END immunoreactivity at P1 and by P14 reaches adult-like proportions of 90% or more of the total B-END immunoreactive material.
采用β-内啡肽（B-END）放射免疫分析法和N-乙酰-β-内啡肽放射免疫分析法，对出生后（P）（第P1、P7、P14、P21、P28、P35、P42天）及成年雄性Sprague-Dawley大鼠的垂体前叶和中间叶提取物进行了分析。在垂体前叶，出生第1天，B-END水平不到成年水平的2%。到出生第42天，该水平已升至成年水平的21%。在中间叶，出生第1天，B-END水平不到成年水平的0.1%，到出生第42天，该水平接近成年水平的45%。从出生第1天到成年期，垂体前叶和中间叶均检测到N-乙酰化B-END。在出生第1天的垂体前叶，N-乙酰-β-内啡肽免疫反应性约占总B-END免疫反应性的25%。到出生第21天，该水平降至10%以下，到出生第42天达到成年水平（低于5%）。另一方面，在中间叶，N-乙酰-β-内啡肽水平在出生第1天占总B-END免疫反应性的70%，到出生第14天达到成年比例，占总B-END免疫反应性物质的90%或更多。
