Cortes R, Supavilai P, Karobath M, Palacios J M
Neurosci Lett. 1983 Dec 11;42(3):249-54. doi: 10.1016/0304-3940(83)90270-7.
The binding of calcium antagonists in the rat hippocampal formation was studied using autoradiography. Hippocampal slices were labeled in vitro with [3H]PN 200-110. High densities of binding sites for calcium antagonists were found in the molecular layer of the dentate gyrus and in the CA3 subfield of the hippocampus. After ablation of the granule cells by local injection of colchicine a marked decrease in the number of [3H]PN 200-110 binding sites density was observed on these areas, while binding to other parts of the hippocampal formation and brain was spared. These results strongly suggest the localization of high densities of calcium channels to the granule cells of the dentate gyrus.
采用放射自显影术研究了钙拮抗剂在大鼠海马结构中的结合情况。用[3H]PN 200 - 110对海马切片进行体外标记。在齿状回分子层和海马CA3亚区发现了高密度的钙拮抗剂结合位点。通过局部注射秋水仙碱损毁颗粒细胞后，在这些区域观察到[3H]PN 200 - 110结合位点密度显著降低，而海马结构和脑的其他部分的结合不受影响。这些结果有力地表明，高密度的钙通道定位于齿状回的颗粒细胞。
