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DNA unwinding ability of Xenopus transcription factor A.

作者信息

Hanas J S, Bogenhagen D F, Wu C W

出版信息

Nucleic Acids Res. 1984 Jan 25;12(2):1265-76. doi: 10.1093/nar/12.2.1265.

DOI:10.1093/nar/12.2.1265
PMID:6320121
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318571/
Abstract

The duplex DNA unwinding ability of Xenopus transcription factor A was examined by treatment of plasmid-protein complexes with calf thymus topoisomerase I and resolution of the topoisomer distributions by agarose gel electrophoresis. Factor A binding to plasmids does result in a small degree of unwinding as evidenced by a shift in topoisomer distributions upon relaxation. Slightly more unwinding by factor A was observed on a specific plasmid, containing four tandemly repeated 5S RNA genes, when compared to a non-specific plasmid of nearly identical size containing no 5S specific sequences. The amount of unwinding observed on the specific plasmid, about one base-pair per gene, suggests that unwinding of the DNA duplex is not an important aspect of binding of factor A to the 5S RNA gene.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e34/318571/3c0390e83bc8/nar00320-0420-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e34/318571/a118257a7936/nar00320-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e34/318571/5c63a470ee5b/nar00320-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e34/318571/3c0390e83bc8/nar00320-0420-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e34/318571/a118257a7936/nar00320-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e34/318571/5c63a470ee5b/nar00320-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e34/318571/3c0390e83bc8/nar00320-0420-a.jpg

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本文引用的文献

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2
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Cell. 1980 Jan;19(1):27-35. doi: 10.1016/0092-8674(80)90385-2.
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A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
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