Pelham H R, Brown D D
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4170-4. doi: 10.1073/pnas.77.7.4170.
5S ribosomal RNA specifically inhibits transcription of cloned repeating units of 5S DNA in a nuclear extract of Xenopus oocytes. The inhibition can be explained by the interaction of 5S RNA with a transcription factor that binds specifically to a control region located within the 5S RNA gene. This transcription factor is identical to an abundant cytoplasmic protein that is known to be complexed with 5S RNA in immature Xenopus oocytes. Thus the presence of large amounts of this protein in these cells can account for both the high rate of synthesis and the subsequent storage of 5S RNA to ribosome synthesis.
5S核糖体RNA能特异性抑制非洲爪蟾卵母细胞核提取物中5S DNA克隆重复单元的转录。这种抑制作用可以通过5S RNA与一种转录因子的相互作用来解释，该转录因子能特异性结合5S RNA基因内的一个控制区域。这种转录因子与一种丰富的细胞质蛋白相同，已知在未成熟的非洲爪蟾卵母细胞中它与5S RNA形成复合物。因此，这些细胞中大量这种蛋白的存在可以解释5S RNA合成速率高以及随后用于核糖体合成的储存现象。
