乙酰胆碱受体的两亲性分析及离子通道的可能形成
Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.
作者信息
Finer-Moore J, Stroud R M
出版信息
Proc Natl Acad Sci U S A. 1984 Jan;81(1):155-9. doi: 10.1073/pnas.81.1.155.
Abstract
Fourier analysis of the hydrophobicities of the acetylcholine receptor subunit sequences reveals regions of amphipathic secondary structure. Prediction of a consensus secondary structure based on this analysis and on an empirical prediction method leads to a testable hypothesis about how the ion channel is formed and might function. Knowledge of the three-dimensional structure of acetylcholine receptors is consistent with features of the model proposed and provides some constraints.
摘要
对乙酰胆碱受体亚基序列疏水性的傅里叶分析揭示了两亲性二级结构区域。基于该分析和一种经验预测方法对共有二级结构进行预测，得出了一个关于离子通道如何形成及可能发挥功能的可检验假设。乙酰胆碱受体三维结构的知识与所提出模型的特征相符，并提供了一些限制条件。
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本文引用的文献
1
Effects of methyl- and ethyl-derivatives of NH4 on the neuromuscular junction.NH4的甲基和乙基衍生物对神经肌肉接头的影响。
Jpn J Physiol. 1959 Jun 25;9(2):130-42. doi: 10.2170/jjphysiol.9.130.
2
Acetylcholine receptor: complex of homologous subunits.乙酰胆碱受体：同源亚基复合体。
Science. 1980 Jun 27;208(4451):1454-6. doi: 10.1126/science.7384786.
3
Structural models of the nicotinic acetylcholine receptor and its toxin-binding sites.烟碱型乙酰胆碱受体及其毒素结合位点的结构模型。
Cell Mol Neurobiol. 1981 Sep;1(3):231-58. doi: 10.1007/BF00710680.
4
Conformational and geometrical properties of beta-sheets in proteins. II. Antiparallel and mixed beta-sheets.蛋白质中β折叠的构象和几何性质。II. 反平行和混合β折叠
J Mol Biol. 1981 Feb 15;146(1):119-41. doi: 10.1016/0022-2836(81)90369-7.
5
Acetylcholine receptor subunits transit a precursor pool before acquiring alpha-bungarotoxin binding activity.乙酰胆碱受体亚基在获得α-银环蛇毒素结合活性之前会经过一个前体池。
J Biol Chem. 1981 Apr 25;256(8):3605-8.
6
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
7
Inhibition of glycosylation with tunicamycin blocks assembly of newly synthesized acetylcholine receptor subunits in muscle cells.用衣霉素抑制糖基化可阻断肌肉细胞中新合成的乙酰胆碱受体亚基的组装。
J Biol Chem. 1982 Mar 10;257(5):2694-701.
8
Structure and function of an acetylcholine receptor.乙酰胆碱受体的结构与功能。
Biophys J. 1982 Jan;37(1):371-83. doi: 10.1016/S0006-3495(82)84685-7.
9
Mechanisms for the incorporation of proteins in membranes and organelles.蛋白质整合到膜和细胞器中的机制。
J Cell Biol. 1982 Jan;92(1):1-22. doi: 10.1083/jcb.92.1.1.
10
In vitro synthesis, glycosylation, and membrane insertion of the four subunits of Torpedo acetylcholine receptor.电鳐乙酰胆碱受体四个亚基的体外合成、糖基化及膜插入
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5598-602. doi: 10.1073/pnas.78.9.5598.
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