Holland J M, Kao J Y, Whitaker M J
Toxicol Appl Pharmacol. 1984 Feb;72(2):272-80. doi: 10.1016/0041-008x(84)90312-0.
An apparatus is described for convenient in vitro quantitation of the rate, extent, and character of living skin penetration by chemicals. Evidence that viability markedly affects epidermal penetration was based upon observations utilizing benzo(a)pyrene. This compound displayed saturation kinetics in the extent of penetration, marked elevation in the rate, and extent of penetration following induction of epidermal mixed function oxidase and negligible penetration of skin previously frozen. These data are interpreted to indicate that for certain compounds, such as benzo(a)pyrene where cutaneous metabolism is expected, meaningful in vitro measures of skin penetration should take into consideration the biochemical viability and metabolic status of the tissue.
描述了一种用于方便地体外定量化学物质对活体皮肤渗透的速率、程度和特性的装置。基于使用苯并(a)芘的观察结果，有证据表明活力显著影响表皮渗透。该化合物在渗透程度上表现出饱和动力学，在表皮混合功能氧化酶诱导后渗透速率和程度显著升高，而先前冷冻的皮肤几乎没有渗透。这些数据被解释为表明，对于某些预期有皮肤代谢的化合物，如苯并(a)芘，有意义的体外皮肤渗透测量应考虑组织的生化活力和代谢状态。
