[Stability of Aujezky's disease virus strain MK25 in an aerosol].

Twenty-two tests were carried out to evaluate the stability of the virus of Aujeszky's disease in aerosol. Use was made of a aerosol chamber of a flow dynamic type, 450 l, of 60 l/min of passing air, 5 min. exposure, 80-100 per cent relative humidity, and temperature range of 14-21.2 degrees C. The size of aerosol particles surpassed 5 micron, and changed according to the composition of the dilution medium of the vaccine. The aerosol was sampled via impinger at the rate of air flow of 5.2 l/min. Tested were the stabilizing properties of 2% glycerin, 10% pepton, 0.8-2.0% saccharose, 2% gelatin, and 5% skimmed milk added to the extender consisting of a 0.2 M phosphate buffer saline, pH 7, specifically for the MK25 strain of the Aujeszky's disease virus in a dispersed status. Highest stability, reaching 11.2% survival rate of the virus in aerosol was recorded at the dispersion of the vaccine in a 0.2 M buffer saline with the addition of 10% pepton and 0.8% saccharose at pH 7.