Münzel P A, Healy D P, Insel P A
Am J Physiol. 1984 Feb;246(2 Pt 2):F240-5. doi: 10.1152/ajprenal.1984.246.2.F240.
We have used autoradiography of [125I]iodocyanopindolol [( 125I]-ICYP) to define the distribution and localization of beta-adrenergic receptors in the rat kidney. [125I]ICYP, a radiolabeled beta-adrenergic antagonist, proved to be an excellent probe to identify these receptors in kidney slices in that specific [125I]ICYP binding was 1) saturable, 2) competed stereoselectively by the agonist isoproterenol and the antagonist propranolol, and 3) competed by agonists in a classical rank order of potency for beta 1-adrenergic receptors (isoproterenol greater than epinephrine congruent to norepinephrine). Autoradiographic studies demonstrated that specific [125I]ICYP sites were present exclusively in the cortex and outer medulla and were localized in glomeruli, ascending limb, and distal tubule-cortical collecting duct. Autoradiography of probes like [125I]ICYP appears to offer a simple and rapid method to assess distribution of adrenergic receptors in the kidney and presumably in other tissues as well.
我们已利用[125I]碘氰吲哚洛尔（[125I]-ICYP）的放射自显影技术来确定大鼠肾脏中β-肾上腺素能受体的分布和定位。[125I]ICYP是一种放射性标记的β-肾上腺素能拮抗剂，被证明是鉴定肾脏切片中这些受体的优良探针，因为特异性的[125I]ICYP结合具有以下特点：1）可饱和；2）被激动剂异丙肾上腺素和拮抗剂普萘洛尔立体选择性竞争；3）被激动剂以β1-肾上腺素能受体的经典效价顺序竞争（异丙肾上腺素＞肾上腺素≡去甲肾上腺素）。放射自显影研究表明，特异性的[125I]ICYP位点仅存在于皮质和外髓质，并定位于肾小球、升支以及远曲小管-皮质集合管。像[125I]ICYP这样的探针的放射自显影似乎提供了一种简单快速的方法来评估肾脏以及可能其他组织中肾上腺素能受体的分布。
