乳腺癌患者肿瘤细胞和淋巴细胞中与小鼠乳腺肿瘤病毒基因组相关的序列。

Sequences related to mouse mammary tumor virus genome in tumor cells and lymphocytes from patients with breast cancer.

作者信息

Crépin M, Lidereau R, Chermann J C, Pouillart P, Magdamenat H, Montagnier L

出版信息

Biochem Biophys Res Commun. 1984 Jan 13;118(1):324-31. doi: 10.1016/0006-291x(84)91104-5.

PMID:6320830

Abstract

Cloned murine mammary tumor virus (MuMTV) sequences allowed us to search for murine mammary tumor virus related sequences in the DNA of surgically removed human breast tumors. Out of 28 tumors so far examined two were found to contain an Eco RI DNA fragment homologous to the long terminal repeat-group antigen (LTR-Gag) and the Envelope (Env) sequences of MuMTV. We have taken the lymphocytes of these patients and cultured them. Rapid growth of lymphocytes, mostly of T origin, occurred in the presence of T cell growth factor (TCGF). Whereas DNA extracted from fresh lymphocytes is negative, that extracted from the 3-day cultured lymphocytes showed MuMTV related sequences. Long term cultures of T cells and a similar culture derived from a healthy person donor were negative at all stages. DNA extracted from the Ebstein Barr Virus-transformed B cells of the patient does not contain the MuMTV related sequences.

摘要

克隆的鼠乳腺肿瘤病毒（MuMTV）序列使我们能够在手术切除的人类乳腺肿瘤DNA中寻找与鼠乳腺肿瘤病毒相关的序列。在迄今为止检测的28个肿瘤中，发现有两个含有与MuMTV的长末端重复-群抗原（LTR-Gag）和包膜（Env）序列同源的Eco RI DNA片段。我们采集了这些患者的淋巴细胞并进行培养。在T细胞生长因子（TCGF）存在的情况下，淋巴细胞（主要是T来源）迅速生长。新鲜淋巴细胞提取的DNA为阴性，而从培养3天的淋巴细胞中提取的DNA显示出与MuMTV相关的序列。T细胞的长期培养以及来自健康供体的类似培养物在所有阶段均为阴性。从患者的爱泼斯坦-巴尔病毒转化的B细胞中提取的DNA不包含与MuMTV相关的序列。