May F E, Westley B R
University Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne, United Kingdom.
Cancer Res. 1989 Jul 15;49(14):3879-83.
Mouse mammary tumour virus (MuMTV) DNA hybridized more strongly to two human EcoRI fragments (6.6 and 9.5 kilobases) in DNA from the MCF-7 human breast cancer cell line than in DNA from normal human placenta. Seven recombinants (NMWV 1E1-7) containing these MuMTV-related sequences were identified. Hybridization of NMWV 1E probes to Southern transfers of human DNA suggested that these probes hybridize to multiple sequences in the human genome. The pattern obtained was very similar to that obtained using MuMTV gag-pol DNA. Analysis of the cloned DNA showed that the NMWV 1E MuMTV-related sequences are arranged as tandem repeats and are contained in EcoRI fragments of 6.6 or 9.5 kilobases. Only two NMWV 1E EcoRI fragments (9.5 and 15 kilobases) were detected in 17 DNA samples prepared from human placenta and blood. In contrast the 6.6-kilobase EcoRI fragment was detected in two (MCF-7 and EFM-19) of seven breast cancer cell lines and the MDA MB-231 cell line contained NMWV 1E sequences in an EcoRI fragment of 9.8 kilobases.
小鼠乳腺肿瘤病毒（MuMTV）DNA与MCF-7人乳腺癌细胞系DNA中的两个人类EcoRI片段（6.6和9.5千碱基）的杂交比与正常人胎盘DNA的杂交更强。鉴定出七个含有这些与MuMTV相关序列的重组体（NMWV 1E1 - 7）。NMWV 1E探针与人DNA的Southern印迹杂交表明这些探针与人类基因组中的多个序列杂交。获得的模式与使用MuMTV gag-pol DNA获得的模式非常相似。对克隆DNA的分析表明，NMWV 1E与MuMTV相关的序列排列为串联重复，并包含在6.6或9.5千碱基的EcoRI片段中。在从人胎盘和血液制备的17个DNA样品中仅检测到两个NMWV 1E EcoRI片段（9.5和15千碱基）。相反，在七个乳腺癌细胞系中的两个（MCF-7和EFM-19）中检测到6.6千碱基的EcoRI片段，并且MDA MB-231细胞系在9.8千碱基的EcoRI片段中含有NMWV 1E序列。
