小鼠乳腺肿瘤病毒相关序列存在于人类DNA中。
Mouse mammary tumour virus related sequences are present in human DNA.
作者信息
May F E, Westley B R, Rochefort H, Buetti E, Diggelmann H
出版信息
Nucleic Acids Res. 1983 Jun 25;11(12):4127-39. doi: 10.1093/nar/11.12.4127.
MuMTV-related sequences have been identified in the DNA of human breast cancer cells using the Southern transfer technique and hybridisation with cloned MuMTV DNA under conditions in which partially mismatched sequences form stable hybrids. Hybridisation with cloned fragments of the MuMTV genome showed that the gag-pol region shares the most homology (estimated to be greater than 80%) with the human MuMTV-related sequences, however, DNA fragments partially homologous to the MuMTV LTR, gag ad env regions were also detected. Analysis of several human DNA samples suggests that the majority of the human MuMTV-related sequences are genetically transmitted but additional Eco R1 fragments were detected in the DNA of one out of three breast cancer cell lines, MCF7. These sequences are potential probes for the human MuMTV-related retroviral sequences and will allow their possible role in human breast cancer to be evaluated.
利用Southern转移技术并在部分错配序列形成稳定杂交体的条件下，将克隆的小鼠乳腺肿瘤病毒（MuMTV）DNA与之杂交，已在人乳腺癌细胞的DNA中鉴定出与MuMTV相关的序列。用MuMTV基因组的克隆片段进行杂交显示，gag-pol区域与人类MuMTV相关序列具有最高的同源性（估计大于80%），然而，也检测到了与MuMTV长末端重复序列（LTR）、gag和env区域部分同源的DNA片段。对几份人类DNA样本的分析表明，大多数人类MuMTV相关序列是通过基因传递的，但在三个乳腺癌细胞系之一的MCF7的DNA中检测到了额外的Eco R1片段。这些序列是人类MuMTV相关逆转录病毒序列的潜在探针，将有助于评估它们在人类乳腺癌中的可能作用。
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