Arai M, Gordon E R, Lieber C S
Biochim Biophys Acta. 1984 Mar 1;797(3):320-7. doi: 10.1016/0304-4165(84)90252-6.
In ethanol-fed baboons, hepatic mitochondrial cytochrome oxidase activity and cytochrome aa3 content were significantly decreased by 58.3 and 50.5%, respectively, compared to their pair-fed controls. However, there was no significant correlation between the two, suggesting that other factors in addition to cytochrome aa3 may be responsible for the depression in cytochrome oxidase activity. The total phospholipid content of the mitochondrial membranes was significantly decreased (0.24 +/- 0.03 mumol of phospholipid phosphorus/mg of protein vs. 0.32 +/- 0.04 in controls). This change was accounted for, in part, by the significant decrease in the levels of phosphatidylcholine and cardiolipin. In addition, the fatty acid pattern of the phospholipids was changed. There was a marked increase in the relative amounts of oleic and linoleic acids and a decrease in arachidonic acid. These changes were associated with an increase in the activity of phospholipase A2. The reactivation rate of phospholipid-depleted cytochrome oxidase by endogenous phospholipids from ethanol-fed baboons was significantly lower than that by phospholipid from pair-fed controls, when measured at an optimal phospholipid to protein ratio. Thus, it appears that alterations in the phospholipid composition of the mitochondrial membranes are responsible, at least in part, for the depression of cytochrome oxidase activity produced by chronic ethanol consumption.
与配对喂养的对照组相比，乙醇喂养的狒狒肝脏线粒体细胞色素氧化酶活性和细胞色素aa3含量分别显著降低了58.3%和50.5%。然而，两者之间没有显著相关性，这表明除细胞色素aa3外的其他因素可能是细胞色素氧化酶活性降低的原因。线粒体膜的总磷脂含量显著降低（磷脂磷0.24±0.03 μmol/毫克蛋白质，而对照组为0.32±0.04）。这种变化部分是由于磷脂酰胆碱和心磷脂水平的显著降低。此外，磷脂的脂肪酸模式发生了变化。油酸和亚油酸的相对含量显著增加，花生四烯酸减少。这些变化与磷脂酶A2活性的增加有关。当以最佳磷脂与蛋白质比例测量时，乙醇喂养的狒狒内源性磷脂对磷脂耗尽的细胞色素氧化酶的再激活率显著低于配对喂养对照组的磷脂。因此，看来线粒体膜磷脂组成的改变至少部分地导致了慢性乙醇消耗所产生的细胞色素氧化酶活性的降低。
