Gorbatenkova E A, Azizova O A, Dubrovin M Iu, Sergienko V I, Khalilov E M
Biull Eksp Biol Med. 1984 Feb;97(2):149-52.
Electron paramagnetic resonance with the use of stearic acid derivatives (5- and 16-doxylstearate) as spin probes was applied to studies of the structural organization of rabbit platelets in experimental atherosclerosis. Substantial differences were established in the molecular packing of phospholipid plasma membranes, associated with a higher molar content of cholesterol in the cells. An increase in the aggregation properties of platelets was also observed, manifesting in a shorter time of the ADP-induced aggregation of platelets isolated from plasma. The data obtained confirm the primary part of membranotropic cholesterol activity in atherosclerosis, attesting to the validity of the "membraneous" hypothesis of the atherogenesis.
利用硬脂酸衍生物（5-和16-二氧硬脂酸盐）作为自旋探针的电子顺磁共振技术被应用于研究实验性动脉粥样硬化中兔血小板的结构组织。结果发现磷脂质细胞膜的分子排列存在显著差异，这与细胞中胆固醇的摩尔含量较高有关。还观察到血小板聚集特性增加，表现为从血浆中分离出的血小板在ADP诱导下聚集的时间缩短。所获得的数据证实了动脉粥样硬化中膜促胆固醇活性的主要作用，证明了动脉粥样硬化发生的“膜性”假说的正确性。
