Vlasova I I, Vakhrusheva T V, Azizova O A, Lopukhin Iu M
Research Institute for Physicochemical Medicine, Moscow.
Vopr Med Khim. 1998 Jan-Feb;44(1):43-54.
The investigation of the effect of oxidized lipoproteins on platelet activity is important for the understanding of the plague formation under atherosclerosis. In the present work, we examined the influence of low density lipoproteins (LDL) on ADP-induced platelet aggregation in the platelet rich plasma. In was demonstrated that mixing of plasma and LDL was accompanied by the decrease of ADP-induced aggregation parameters as compared to control (mixing with buffer). After 1 h incubation, platelet ADP-aggregation in the sample containing oxidized LDL (oxLDL) exceeded the ADP-aggregation in the control sample. The dependence of the aggregation parameters on the incubation time and on the degree of LDL oxidation were obtained. No difference in the cholesterol and phospholipid content was observed between cells incubated with buffer, native or oxidized LDL. Therefore, the possible oxLDL-induced accumulation of cholesterol in platelet membranes is excluded as a reason for the increased cell aggregation.
研究氧化型脂蛋白对血小板活性的影响，对于理解动脉粥样硬化下的斑块形成具有重要意义。在本研究中，我们检测了低密度脂蛋白（LDL）对富含血小板血浆中ADP诱导的血小板聚集的影响。结果表明，与对照组（与缓冲液混合）相比，血浆与LDL混合后，ADP诱导的聚集参数降低。孵育1小时后，含有氧化型LDL（oxLDL）的样品中的血小板ADP聚集超过了对照样品中的ADP聚集。获得了聚集参数与孵育时间以及LDL氧化程度的相关性。在用缓冲液、天然LDL或氧化型LDL孵育的细胞之间，未观察到胆固醇和磷脂含量的差异。因此，排除了oxLDL诱导的胆固醇在血小板膜中积累作为细胞聚集增加原因的可能性。
