Mora F, Lee T F, Myers R D
Brain Res Bull. 1983 Nov;11(5):613-6. doi: 10.1016/0361-9230(83)90132-6.
The effect of the central blockade of alpha- and beta-adrenoreceptors on the action of norepinephrine (NE) infused by the intracerebroventricular (ICV) route was examined in the unanesthetized primate. After cannulae for ICV infusion were implanted sterotaxically, each monkey (Macaca fasicularis) was placed in a restraint chair fitted with a transparent dome for measurement of O2 uptake. During an experiment, the following responses were monitored simultaneously on a polygraph: skin and colonic temperatures, heart and respiratory rates, and metabolic rates as reflected by O2 uptake. Following an ICV infusion of 150 micrograms NE, colonic temperature, heart and metabolic rates declined while skin temperature increased and respiratory rate remained unchanged. Pretreatment with ICV phentolamine (100 micrograms), an alpha-adrenoreceptor antagonist, attenuated the changes in temperature, heart and metabolic rates and reduced respiratory rate. Similar ICV infusion of 100 micrograms propranolol, a beta-adrenoreceptor antagonist, tended also to attenuate the responses induced by NE, but overall was much less efficacious than phentolamine. Our results indicate that although catecholaminergic neurons mediate these vital physiological processes principally by central alpha-adrenoreceptors, a substantial beta-adrenoreceptor component also participates in the mechanism responsible for specific functional changes.
在未麻醉的灵长类动物中，研究了脑室内（ICV）注入去甲肾上腺素（NE）时，中枢α和β肾上腺素能受体阻断对其作用的影响。通过立体定位植入ICV注入套管后，将每只猕猴（食蟹猴）置于装有透明圆顶的约束椅中，以测量氧气摄取量。在实验过程中，用多导记录仪同时监测以下反应：皮肤和结肠温度、心率和呼吸频率，以及由氧气摄取量反映的代谢率。ICV注入150微克NE后，结肠温度、心率和代谢率下降，而皮肤温度升高，呼吸频率保持不变。用α肾上腺素能受体拮抗剂ICV酚妥拉明（100微克）预处理可减弱温度、心率和代谢率的变化，并降低呼吸频率。类似地，ICV注入100微克β肾上腺素能受体拮抗剂普萘洛尔也倾向于减弱NE诱导的反应，但总体效果远不如酚妥拉明。我们的结果表明，尽管儿茶酚胺能神经元主要通过中枢α肾上腺素能受体介导这些重要的生理过程，但大量的β肾上腺素能受体成分也参与了负责特定功能变化的机制。
