Adrian M, Dubochet J, Lepault J, McDowall A W
Nature. 1984;308(5954):32-6. doi: 10.1038/308032a0.
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.
未固定、未染色且无支撑的病毒悬液的薄玻璃化层可在易于控制的条件下制备，用于冷冻电子显微镜观察。病毒颗粒似乎没有因脱水、冷冻或吸附到支撑物上而造成的那种损伤，而在为传统电子显微镜制备生物样品时会遇到这些问题。玻璃化标本的冷冻电子显微镜为高分辨率观察提供了可能性，与任何其他电子显微镜方法相比都毫不逊色。
