Olson N H, Baker T S
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Ultramicroscopy. 1989 Jul-Aug;30(3):281-97. doi: 10.1016/0304-3991(89)90057-0.
The diameters of several frozen-hydrated, spherical viruses were determined using polyoma virus as either an external or an internal calibration standard. The methods described provide a reproducible and accurate way to calibrate microscope magnification. The measured diameters are in excellent agreement with respective measurements previously reported for aqueous samples at room temperature using X-ray diffraction methods. These results indicate that the native morphology and dimensions of biological macromolecules are better preserved in frozen-hydrated samples when compared with more conventional electron microscopy techniques such as negative-staining, metal shadowing or thin-sectioning.
使用多瘤病毒作为外部或内部校准标准，测定了几种冷冻水合球形病毒的直径。所描述的方法提供了一种可重复且准确的校准显微镜放大倍数的方法。测得的直径与先前使用X射线衍射方法在室温下对水性样品进行的相应测量结果非常吻合。这些结果表明，与负染色、金属阴影或超薄切片等更传统的电子显微镜技术相比，生物大分子的天然形态和尺寸在冷冻水合样品中得到了更好的保留。
