Edmonds P D, Sancier K M
Ultrasound Med Biol. 1983 Nov-Dec;9(6):635-9. doi: 10.1016/0301-5629(83)90009-1.
The statement is often made that free radicals will be produced during the violent collapse of transient cavities in liquids, that may result from the passage of ultrasonic waves in the low megahertz frequency range. Evidence for such free radicals is mostly indirect. We have insonated some argon-saturated aqueous solutions of biological molecules (trypsin, Dulbecco's Minimum Essential Medium, fetal bovine serum, and human blood plasma) and whole human blood, all containing a spin trapping compound, at 1 MHz, continuous wave, 6.6 W/cm2 (SPTA) for 15 min at 4-8 degrees C. Electron spin resonance spectra of the samples were recorded 10 and 30 min. after cessation of insonation. The spectra included those of spin adducts formed with .OH and .H radicals, which were absent in the cases of the sham treated controls. Thus, we have demonstrated the formation of the highly reactive .OH and .H radicals in the insonated media, even in the presence of natural radical scavengers in the mammalian derived products.
常有人指出，在液体中瞬时空腔剧烈坍塌期间会产生自由基，这可能是由低兆赫兹频率范围内的超声波通过所致。此类自由基的证据大多是间接的。我们在4至8摄氏度下，以1兆赫兹连续波、6.6瓦/平方厘米（声功率）对一些含有自旋捕获化合物的生物分子（胰蛋白酶、杜尔贝科改良伊格尔培养基、胎牛血清和人血浆）的氩饱和水溶液以及全血进行了15分钟的超声处理。在停止超声处理10分钟和30分钟后记录了样品的电子自旋共振光谱。光谱中包括与·OH和·H自由基形成的自旋加合物的光谱，而在假处理对照的情况下则不存在这些光谱。因此，我们已经证明，即使在哺乳动物衍生产品中存在天然自由基清除剂的情况下，在超声处理的介质中也会形成高反应性的·OH和·H自由基。
