Polack A, Delius H, Zimber U, Bornkamm G W
Virology. 1984 Feb;133(1):146-57. doi: 10.1016/0042-6822(84)90433-1.
The Epstein-Barr virus genome carried in the Burkitt lymphoma nonproducer cell line Raji was characterized by partial denaturation mapping and by hybridization of cloned viral fragments to filters containing separated Raji DNA fragments. Partial denaturation mapping revealed that the EBV DNA population of Raji cells is homogeneous and that two deletions are observed in distant parts of the genome compared to linear DNA isolated from virus particles of different strains. These deletions were characterized by blot analysis. One deletion of 3.15 kb lies within HindIII-E; the second is 2.4 kb and is located close to the right terminus of linear viral DNA. The two deletions were observed in several cell lines derived from the Raji line. These deletions might contribute to the inability of Raji cells to produce EBV either spontaneously or upon induction.
携带于伯基特淋巴瘤非生产细胞系Raji中的爱泼斯坦-巴尔病毒基因组,通过部分变性图谱以及克隆的病毒片段与含有分离的Raji DNA片段的滤膜杂交进行表征。部分变性图谱显示,Raji细胞的EBV DNA群体是同质的,并且与从不同菌株的病毒颗粒中分离出的线性DNA相比,在基因组的远距离部分观察到两个缺失。通过印迹分析对这些缺失进行了表征。一个3.15 kb的缺失位于HindIII-E内;第二个缺失为2.4 kb,位于线性病毒DNA的右端附近。在源自Raji系的几个细胞系中都观察到了这两个缺失。这些缺失可能导致Raji细胞无法自发或在诱导后产生EBV。