Bornkamm G W, Delius H, Zimber U, Hudewentz J, Epstein M A
J Virol. 1980 Sep;35(3):603-18. doi: 10.1128/JVI.35.3.603-618.1980.
Epstein-Barr virus (EBV) originating from Burkitt's lymphoma (P3HR-1 and CC34-5), nasopharyngeal carcinoma (M-ABA), transfusion mononucleosis (B95-8), and a patient with acute myeloblastic leukemia (QIMR-WIL) was isolated from virus-carrying lymphoid cell lines after induction with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. Viral DNA was analyzed by partial denaturation mapping and by use of the restriction endonucleases EcoRI, HindIII, and SalI and separation of fragments in 0.4% agarose. By using the restriction enzyme data of B95-8 (EBV) and W91 (EBV) obtained by Given and Kieff (D. Given and E. Kieff, J. Virol. 28:524-542, 1978), maps were established for the other virus strains. Comigrating fragments were assumed to be identical or closely related among the different strains. Fragments of different strains migrating differently were isolated, purified, radioactively labeled, and mapped by hybridization against blots of separated viral fragments. The results were as follows. (i) All strains studied were closely related. (ii) The number of internal repeats was variable among and within viral strains. (iii) B95-8 (EBV) was the only strain with a large deletion of about 12,000 base pairs at the right-hand side of the molecule. At the same site, small deletions of about 400 to 500 base pairs were observed in P3HR-1 (EBV) and M-ABA (EBV) DNA. (iv) P3HR-1 (EBV), the only nontransforming EBV strain, had a deletion of about 3,000 to 4,000 base pairs in the long unique region adjacent to the internal repeats carrying a HindIII site. (v) Small inserted sequences of 150 to 400 base pairs were observed in M-ABA (EBV) and B95-8 (EBV) at identical sites in the middle of the long unique region. (vi) Near this site, an insertion of about 1,000 base pairs was found in P3HR-1 (EBV) DNA. (vii) The cleavage patterns of P3HR-1 virus DNA and the results of blot hybridizations with P3HR-1 virus fragments are not conclusive and point to the possibility that in addition to the normal cleavage pattern some viral sequences may be arranged differently. Even though it is possible that small differences in the genome organization may have significant biological effects, the great similarity among different EBV strains does not favor the hypothesis that disease-specific subtypes exist.
源自伯基特淋巴瘤（P3HR - 1和CC34 - 5）、鼻咽癌（M - ABA）、输血后单核细胞增多症（B95 - 8）以及一名急性髓细胞白血病患者（QIMR - WIL）的爱泼斯坦 - 巴尔病毒（EBV），在用肿瘤启动子12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯诱导后，从携带病毒的淋巴样细胞系中分离出来。通过部分变性图谱分析以及使用限制性内切酶EcoRI、HindIII和SalI并在0.4%琼脂糖中分离片段来分析病毒DNA。利用Given和Kieff（D. Given和E. Kieff，《病毒学杂志》28:524 - 542，1978）获得的B95 - 8（EBV）和W91（EBV）的限制性酶切数据，为其他病毒株构建图谱。假定不同病毒株中共同迁移的片段是相同的或密切相关的。分离出不同病毒株中迁移情况不同的片段，进行纯化、放射性标记，并通过与分离的病毒片段印迹杂交进行图谱分析。结果如下：（i）所研究的所有病毒株密切相关。（ii）病毒株之间以及同一病毒株内部的内部重复序列数量是可变的。（iii）B95 - 8（EBV）是唯一在分子右侧有大约12,000个碱基对大缺失的病毒株。在同一位置，在P3HR - 1（EBV）和M - ABA（EBV）DNA中观察到大约400至500个碱基对的小缺失。（iv）P3HR - 1（EBV）是唯一的非转化性EBV病毒株，在与携带HindIII位点的内部重复序列相邻的长单一区域中有大约3,000至4,000个碱基对的缺失。（v）在M - ABA（EBV）和B95 - 8（EBV）的长单一区域中间的相同位置观察到150至400个碱基对的小插入序列。（vi）在该位置附近，在P3HR - 1（EBV）DNA中发现了大约1,000个碱基对的插入。（vii）P3HR - 1病毒DNA的切割模式以及与P3HR - 1病毒片段的印迹杂交结果并不确凿，这表明除了正常的切割模式外，一些病毒序列可能有不同的排列方式。尽管基因组组织中的小差异可能具有显著的生物学效应，但不同EBV病毒株之间的高度相似性并不支持存在疾病特异性亚型的假说。
