Borbolla M G, Rosen B P
Arch Biochem Biophys. 1984 Feb 15;229(1):98-103. doi: 10.1016/0003-9861(84)90134-6.
When energy-starved cells of Escherichia coli were passively loaded with 22Na+, efflux of sodium could be initiated by addition of a source of metabolic energy. Conditions were established where the source of energy was phosphate bond energy, an electrochemical proton gradient, or both. Only an electrochemical proton gradient was required for efflux from intact cells. These results are consistent with secondary exchange of Na+ for H+ catalyzed by a sodium/proton antiporter.
当能量匮乏的大肠杆菌细胞被动加载22Na+时，添加代谢能量来源可引发钠的外流。建立了能量来源为磷酸键能、电化学质子梯度或两者兼有的条件。完整细胞外流仅需电化学质子梯度。这些结果与钠/质子反向转运体催化的Na+与H+的二次交换一致。
