Folkers K, Lu Y A, Rosell S
Biochem Biophys Res Commun. 1984 Jan 30;118(2):405-8. doi: 10.1016/0006-291x(84)91317-2.
Two novel neuropeptides, neurokinin alpha and beta isolated from porcine spinal cord, were announced. We have synthesized neurokinin alpha as Asp-Met-His-Asp-Phe-Phe-Val-Gly-Leu-Met-NH2, which were 98-99% pure by HPLC. Assays on the isolated guinea pig ilium showed neurokinin alpha to have 81% and neurokinin beta to have 65% of the activity of Substance P. Knowledge of these three related peptides having a common activity opens new considerations of their intrinsic physiological roles in neurotransmission versus pharmacological activities, and reappraisal of the diverse activities of Substance P including that in the inflammatory response of the eye.
从猪脊髓中分离出两种新型神经肽，即神经激肽α和β。我们已合成神经激肽α，其序列为天冬氨酸-甲硫氨酸-组氨酸-天冬氨酸-苯丙氨酸-苯丙氨酸-缬氨酸-甘氨酸-亮氨酸-甲硫氨酸-氨基，通过高效液相色谱法测定其纯度为98 - 99%。对分离出的豚鼠回肠进行的检测显示，神经激肽α具有P物质81%的活性，神经激肽β具有P物质65%的活性。这三种具有共同活性的相关肽的发现，为它们在神经传递中的内在生理作用与药理活性方面开启了新的思考，同时也促使人们重新评估P物质的多种活性，包括其在眼部炎症反应中的活性。
