Stanislawsky L, Mongiat F, Moura Neto V
Biochem Biophys Res Commun. 1984 Jan 30;118(2):580-6. doi: 10.1016/0006-291x(84)91342-1.
A 43K protein present in avian myeloblastosis virus has been identified as actin by 2D gel electrophoresis and peptide mapping proteolysis. Electron microscopy of chicken embryo fibroblasts infected with different pseudotypes of oncornaviruses treated with anti-actin antibody showed positive staining at the level of the virions especially on buds. Our results indicate that this actin is unlikely to have been artefactually absorbed at the virion surface during its preparation. It may therefore play a possible role in the budding of enveloped virions.
通过二维凝胶电泳和肽图谱蛋白酶解分析，已确定禽成髓细胞瘤病毒中存在的一种43K蛋白为肌动蛋白。用抗肌动蛋白抗体处理感染了不同假型肿瘤病毒的鸡胚成纤维细胞，电子显微镜观察显示，在病毒粒子水平尤其是芽处有阳性染色。我们的结果表明，这种肌动蛋白不太可能在制备过程中被人为吸附在病毒粒子表面。因此，它可能在包膜病毒粒子的出芽过程中发挥作用。
