De Bortoli M, Giunta C, Dati C, Usseglio L
Boll Soc Ital Biol Sper. 1984 Jan 30;60(1):71-7.
There is great interest in the histologic localization of the Na, K-pump in various tissues: histochemical methods in this case work poorly, so a specific antibody against a purified soluble Na, K-ATPase is necessary. We approached this problem with a two-step ionic detergent treatment; the separation of the solubilized enzyme was attempted by gel filtration and ion-exchange chromatography. The gel filtration purified form was active and nearly pure, while the ion-exchange on was purer but inactive. PAGE analyses of the various enzyme forms are presented.
在这种情况下，组织化学方法效果不佳，因此需要一种针对纯化的可溶性钠钾ATP酶的特异性抗体。我们采用两步离子去污剂处理方法来解决这个问题；尝试通过凝胶过滤和离子交换色谱法分离溶解的酶。凝胶过滤纯化形式具有活性且近乎纯净，而离子交换形式更纯但无活性。文中给出了各种酶形式的聚丙烯酰胺凝胶电泳分析结果。
