Giunta C, de Bortoli M
Boll Soc Ital Biol Sper. 1979 Jul 30;55(14):1374-80.
Microsomal preparations with Na+/K+-dependent ATPase activity from the outer medulla of rabbit and pig kidney were obtained. Purifications were assaied by centrifugation on sucrose discontinuous gradients and gel-filtration on Sepharose 6B, after detergent incubation. Sodium dodecylsulfate, with ATP as protecting agent, can remove a maximal amount of non specific proteins from the membranes and allows the recovery of a fraction with very high specific activity. The approach to purification by affinity chromatography techniques leads to interesting results, which induce us to pursue the present researchs to applicate an affinity method to Na+/K+-ATPase.
从兔和猪肾外髓质获得了具有Na⁺/K⁺依赖性ATP酶活性的微粒体制剂。在去污剂孵育后，通过在蔗糖不连续梯度上离心和在琼脂糖6B上进行凝胶过滤来测定纯化情况。以ATP作为保护剂的十二烷基硫酸钠可以从膜上去除最大量的非特异性蛋白质，并能回收具有非常高比活性的部分。通过亲和色谱技术进行纯化的方法产生了有趣的结果，这促使我们继续目前的研究，以便将亲和方法应用于Na⁺/K⁺-ATP酶。
