基于噬菌体M13的新型通用克隆和测序载体。
New versatile cloning and sequencing vectors based on bacteriophage M13.
作者信息
Kieny M P, Lathe R, Lecocq J P
出版信息
Gene. 1983 Dec;26(1):91-9. doi: 10.1016/0378-1119(83)90039-2.
A new pair of cloning and sequencing vectors based on bacteriophage M13mp7 has been developed. These vectors (M13tg130 and M13tg131) contain, in addition to the EcoRI, BamHI, HindIII, SmaI, SalI and PstI sites present in other vectors [cf., M13mp8 and M13mp9, Messing and Vieira, Gene 19 (1982) 269-276], unique restriction recognition sequences for the enzymes EcoRV, KpnI, SphI, SstI and XbaI. A restriction site for the enzyme BglII has been incorporated into the polylinker region of one of the vector pair to permit rapid discrimination between the two vectors.
基于噬菌体M13mp7开发了一对新的克隆和测序载体。除了其他载体（参见M13mp8和M13mp9，Messing和Vieira，《基因》19卷，第269 - 276页，1982年）中存在的EcoRI、BamHI、HindIII、SmaI、SalI和PstI位点外，这些载体（M13tg130和M13tg131）还含有EcoRV、KpnI、SphI、SstI和XbaI酶的独特限制性识别序列。载体对中的一个载体的多克隆位点区域引入了BglII酶的限制性位点，以便快速区分这两个载体。
Zotero 插件