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二十一种卡隆载体噬菌体的限制酶切图谱。

Restriction maps for twenty-one Charon vector phages.

作者信息

de Wet J R, Daniels D L, Schroeder J L, Williams B G, Denniston-Thompson K, Moore D D, Blattner F R

出版信息

J Virol. 1980 Jan;33(1):401-10. doi: 10.1128/JVI.33.1.401-410.1980.

DOI:10.1128/JVI.33.1.401-410.1980
PMID:6245241
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC288556/
Abstract

The mapping of the sites of cleavage of nine restriction endonucleases (EcoRI, HindIII, BamHI, SalI, KpnI, SstI, BglII, XhoI, and XbaI) on 21 Charon phage vectors is described. Maps of individual subsections were obtained and then combined to assemble the complete vector maps. Calculations of maximum and minimum sizes of inserts which may be carried by the vectors using different restriction endonucleases or pairs of restriction endonucleases are presented. The regions mapped include several parts of phi 80 that had not been mapped previously.

摘要

本文描述了9种限制性内切酶(EcoRI、HindIII、BamHI、SalI、KpnI、SstI、BglII、XhoI和XbaI)在21种Charon噬菌体载体上的切割位点图谱。获得了各个子部分的图谱,然后将它们组合起来以构建完整的载体图谱。文中给出了使用不同的限制性内切酶或限制性内切酶对时,载体可能携带的插入片段的最大和最小尺寸的计算结果。所绘制图谱的区域包括之前未绘制过的φ80的几个部分。

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本文引用的文献

1
New map of bacteriophage lambda DNA.噬菌体λDNA的新图谱。
J Virol. 1980 Jan;33(1):390-400. doi: 10.1128/JVI.33.1.390-400.1980.
2
Deletions and insertions in the immunity region of coliphage lambda: revised measurement of the promoter-startpoint distance.λ噬菌体免疫区域中的缺失和插入:启动子-起始点距离的修订测量
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Hybridization of labeled RNA to DNA in agarose gels.标记RNA与琼脂糖凝胶中的DNA杂交。
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A simple method for the preparation of large quantities of pure plasmid DNA.一种制备大量纯质粒DNA的简单方法。
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Physical structure of the replication origin of bacteriophage lambda.噬菌体λ复制起点的物理结构
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6
Construction of chimeric phages and plasmids containing the origin of replication of bacteriophage lambda.构建含有噬菌体λ复制起点的嵌合噬菌体和质粒。
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7
Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.卡戎噬菌体:用于DNA克隆的λ噬菌体更安全的衍生物。
Science. 1977 Apr 8;196(4286):161-9. doi: 10.1126/science.847462.
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Least-squares method for restriction mapping.用于限制酶切图谱分析的最小二乘法
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9
Adjacent insertion sequences IS2 and IS5 in bacteriophage Mu mutants and an IS5 in a lambda darg bacteriophage.噬菌体 Mu 突变体中的相邻插入序列 IS2 和 IS5 以及 λdarg 噬菌体中的一个 IS5。
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10
Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning.用于DNA克隆的杂交噬菌体λCharon载体的构建与特性分析。
J Virol. 1979 Feb;29(2):555-75. doi: 10.1128/JVI.29.2.555-575.1979.