de Wet J R, Daniels D L, Schroeder J L, Williams B G, Denniston-Thompson K, Moore D D, Blattner F R
J Virol. 1980 Jan;33(1):401-10. doi: 10.1128/JVI.33.1.401-410.1980.
The mapping of the sites of cleavage of nine restriction endonucleases (EcoRI, HindIII, BamHI, SalI, KpnI, SstI, BglII, XhoI, and XbaI) on 21 Charon phage vectors is described. Maps of individual subsections were obtained and then combined to assemble the complete vector maps. Calculations of maximum and minimum sizes of inserts which may be carried by the vectors using different restriction endonucleases or pairs of restriction endonucleases are presented. The regions mapped include several parts of phi 80 that had not been mapped previously.
本文描述了9种限制性内切酶(EcoRI、HindIII、BamHI、SalI、KpnI、SstI、BglII、XhoI和XbaI)在21种Charon噬菌体载体上的切割位点图谱。获得了各个子部分的图谱,然后将它们组合起来以构建完整的载体图谱。文中给出了使用不同的限制性内切酶或限制性内切酶对时,载体可能携带的插入片段的最大和最小尺寸的计算结果。所绘制图谱的区域包括之前未绘制过的φ80的几个部分。