赋予对潮霉素B抗性的大肠杆菌K-12基因在浅青紫链霉菌中的表达。
Expression in Streptomyces ambofaciens of an Escherichia coli K-12 gene which confers resistance to hygromycin B.
作者信息
Kuhstoss S, Rao R N
出版信息
Gene. 1983 Dec;26(2-3):295-9. doi: 10.1016/0378-1119(83)90200-7.
PMID:6323267
Abstract
We have constructed two plasmid vectors (pKC293 and pKC305) that can replicate in Escherichia coli K-12 and Streptomyces ambofaciens. These shuttle vectors were used to demonstrate the expression of two E. coli genes, hygromycin B (Hm) resistance and Tn5 neomycin (Nm) resistance, in S. ambofaciens.
摘要
我们构建了两种质粒载体（pKC293和pKC305），它们能够在大肠杆菌K-12和栖土曲霉中复制。这些穿梭载体用于证明两种大肠杆菌基因，潮霉素B（Hm）抗性和Tn5新霉素（Nm）抗性，在栖土曲霉中的表达。
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