Strannegård O, Strannegård I L
Int Arch Allergy Appl Immunol. 1984;74(1):9-14. doi: 10.1159/000233508.
The cyclic AMP (cAMP)-elevating substances dibutyryl-cAMP (dbcAMP), isoproterenol and theophylline were found to suppress the spontaneous in vitro IgE synthesis of peripheral blood mononuclear cells (MNC) from patients with atopic dermatitis, when added at high concentrations (10(-3) -10(-4) M) to the IgE-producing cell cultures. In contrast, low concentrations of the substances (10(-8) -10(-12) M) significantly enhanced IgE production. This enhancement was probably due to effects of cAMP on T cells since pretreatment of allogeneic MNC or T cells with dbcAMP abrogated their suppressive effect or resulted in enhancement of IgE synthesis in coculture experiments. Likewise, pretreated T cells from atopics stimulated the IgE production of autologous B cells more than did untreated T cells. These findings may possibly have bearing on the pathogenesis of the atopic diseases, which are associated with abnormalities of the cyclic nucleotide metabolism.
发现环磷酸腺苷（cAMP）升高物质二丁酰环磷腺苷（dbcAMP）、异丙肾上腺素和茶碱在高浓度（10⁻³ - 10⁻⁴M）添加到产生IgE的细胞培养物中时，可抑制特应性皮炎患者外周血单个核细胞（MNC）的体外自发IgE合成。相反，低浓度的这些物质（10⁻⁸ - 10⁻¹²M）可显著增强IgE产生。这种增强可能是由于cAMP对T细胞的作用，因为在共培养实验中，用dbcAMP预处理同种异体MNC或T细胞可消除其抑制作用或导致IgE合成增强。同样，来自特应性个体的预处理T细胞比未处理的T细胞更能刺激自体B细胞产生IgE。这些发现可能与特应性疾病的发病机制有关，特应性疾病与环核苷酸代谢异常有关。
