Kimata H, Yoshida A, Ishioka C, Mikawa H
Department of Paediatrics, Kyoto University Hospital, Japan.
Clin Exp Immunol. 1991 Mar;83(3):483-7. doi: 10.1111/j.1365-2249.1991.tb05665.x.
Recombinant human erythropoietin enhanced spontaneous IgE production (200-300% enhancement) in cultures of peripheral blood mononuclear cells (MNC) from atopic patients. In contrast, IgG and IgA production were only slightly enhanced (30-50% enhancement), and IgM production was not affected by erythropoietin. The enhancement of IgE production by erythropoietin was indirect since it required T cells and monocytes. However, erythropoietin effect was specific since enhancement was blocked by anti-erythropoietin antibody but not by control antibody. Interleukin-4 (IL-4) also enhanced spontaneous IgE production from atopic MNC. However, the enhancing effect by erythropoietin is different from that by IL-4, since the erythropoietin effect was not blocked by anti-IL-4 antibody, and conversely IL-4 effect was not blocked by anti-erythropoietin antibody. In contrast to the enhancing effect on atopic MNC, erythropoietin failed to induce IgE production in cultures of MNC from normal donors while IL-4 induced IgE production from normal MNC. However, when normal MNC were pre-incubated with IL-4, erythropoietin enhanced IgE production from IL-4-pre-incubated MNC. Moreover, B cells separated from IL-4-pre-incubated MNC produced IgE which was enhanced by erythropoietin. However, this effect required T cells and monocytes. These results indicate that erythropoietin could regulate ongoing IgE production in vitro by T cell- and monocyte-dependent mechanisms.
重组人促红细胞生成素可增强特应性患者外周血单个核细胞（MNC）培养物中自发性IgE的产生（增强200 - 300%）。相比之下，IgG和IgA的产生仅略有增强（增强30 - 50%），而IgM的产生不受促红细胞生成素的影响。促红细胞生成素对IgE产生的增强作用是间接的，因为它需要T细胞和单核细胞。然而，促红细胞生成素的作用具有特异性，因为其增强作用被抗促红细胞生成素抗体阻断，而未被对照抗体阻断。白细胞介素-4（IL-4）也可增强特应性MNC自发性IgE的产生。然而，促红细胞生成素的增强作用与IL-4不同，因为促红细胞生成素的作用未被抗IL-4抗体阻断，反之，IL-4的作用也未被抗促红细胞生成素抗体阻断。与对特应性MNC的增强作用相反，促红细胞生成素未能在正常供体的MNC培养物中诱导IgE产生，而IL-4可诱导正常MNC产生IgE。然而，当正常MNC与IL-4预孵育后，促红细胞生成素可增强来自IL-4预孵育MNC的IgE产生。此外，从IL-4预孵育MNC中分离出的B细胞产生的IgE可被促红细胞生成素增强。然而，这种作用需要T细胞和单核细胞。这些结果表明，促红细胞生成素可通过T细胞和单核细胞依赖的机制在体外调节正在进行的IgE产生。
