Amundsen S K, Parris D S
J Virol Methods. 1984 Feb;8(1-2):19-25. doi: 10.1016/0166-0934(84)90037-5.
Intertypic recombination between herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) was detected using DNA from mixedly infected cells. Because HSV-1 and HSV-2 share a 50% base sequence homology along the genome but have markedly different DNA restriction enzyme cleavage patterns, recombination events can be detected and quantified by analysis of restriction endonuclease digests for the presence of novel DNA fragments. We have used this technique to quantify the degree of interference by HSV-2 on HSV-1 replication as well as the effect of limiting the availability of one genome on the frequency of intertypic recombination. Because this technique does not require production of viable progeny virions, it should also be useful for studying early recombination events.
利用来自混合感染细胞的DNA检测了1型和2型单纯疱疹病毒（HSV-1和HSV-2）之间的型间重组。由于HSV-1和HSV-2在整个基因组中具有50%的碱基序列同源性，但具有明显不同的DNA限制性内切酶切割模式，因此可以通过分析限制性内切酶消化产物中是否存在新的DNA片段来检测和定量重组事件。我们已使用该技术来量化HSV-2对HSV-1复制的干扰程度，以及限制一个基因组的可用性对型间重组频率的影响。由于该技术不需要产生有活力的子代病毒粒子，因此它也应有助于研究早期重组事件。
